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feeding · contextual evidence

Stylophora: prey nitrogen under heat stress

Stylophora pistillata · Artemia salina

Names as reported by the authors; no accepted-taxon or specimen-identity claim.

Reading the evidence

These records describe traits and results under specific study conditions. They do not establish recommended aquarium settings.

What the study tells us

Lower labeled-prey nitrogen assimilation during bleaching does not mean all dissolved nutrient uptake changed. This experiment does not compare retail feeds or establish feeding as a universal heat-stress rescue.

Study conditions

Seventy-two fragments from six mother colonies, four aquaria (two per temperature); 25 degrees C versus ramp to 32 degrees C at 1 degree per 3.5 days, sampled three days after reaching 32. About 200 micromol photons m−2 s−1; Artemia at repletion twice weekly. Isotope-labeled prey and dissolved substrates, host/symbiont separation, amino-acid-specific isotopes and oxygen flux assays.

Methods and evidence class

controlled captive experiment

Oxygen exchange, symbiont counts and labeled Artemia/dissolved-substrate assays; separate host and symbiont fractions. Mixed-effects and PERMANOVA models accounted for aquarium/colony.

Findings

obs-pmc11481558-01

At 32 degrees C, symbiont density declined by more than 80% (p less than 0.001) and respiration increased (p=0.001); the approximately 50% decline in net photosynthesis was not statistically significant at 0.05 (p=0.088).

Measurements and uncertainty
  • {"metric": "symbiont density decrease", "uncertainty": "P<0.001", "unit_as_published": "%", "value_as_published": "more than 80%"}
Where to find it in the paper

RESULTS | ./body/sec[3]/p[1]

Read the cited article ↗

obs-pmc11481558-02

Six hours after labeled Artemia feeding, nitrogen assimilation was approximately threefold higher in hosts than symbionts at both temperatures, but assimilation rates were significantly lower at 32 degrees C (p=0.005). This is not evidence that warming increases prey uptake.

Measurements and uncertainty
  • {"metric": "host versus symbiont nitrogen assimilation", "uncertainty": "temperature comparison P=0.005", "unit_as_published": "fold comparison", "value_as_published": "three times higher"}
Where to find it in the paper

RESULTS | ./body/sec[3]/p[3]

Read the cited article ↗

Limitations and source dependence

  • Only two tanks per temperature and three fragments per isotope sampling time; short terminal hot exposure.
  • The assay used a source-reported concentration of approximately 2000 Artemia per mL. This experimental concentration is not an aquarium feeding recommendation.
  • The reduction in net photosynthesis was not statistically significant (p=0.088), despite stronger wording in the Discussion.
  • Isotope allocation and uptake are not a feeding-frequency or recovery trial; increased dietary reliance can coexist with reduced assimilation.

All isotope tracers and physiological endpoints share one heat-exposure cohort. Potential shared Monaco stock provenance with PMC7285240 is not resolved as independent genotypes.

Evidence family: study-pmc11481558

Method passage locators

MATERIAL AND METHODS / Experimental design | ./body/sec[2]/sec[1]/p[1]

MATERIAL AND METHODS / Experimental design | ./body/sec[2]/sec[1]/p[2]

MATERIAL AND METHODS / Experimental design | ./body/sec[2]/sec[1]/p[3]

MATERIAL AND METHODS / Experimental design | ./body/sec[2]/sec[1]/p[4]

MATERIAL AND METHODS / Experimental design | ./body/sec[2]/sec[1]/p[5]

MATERIAL AND METHODS / Statistics | ./body/sec[2]/sec[5]/p[1]

Source and citation

Unveiling the importance of heterotrophy for coral symbiosis under heat stress ↗

Martinez S, Grover R, Ferrier-Pagès C. (2024). Unveiling the importance of heterotrophy for coral symbiosis under heat stress. DOI: 10.1128/mbio.01966-24. Copyright © 2024 Martinez et al.This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.

DOI: 10.1128/mbio.01966-24

CC-BY-4.0 · Original summaries and structured facts adapted by Reefory.

Source retrieval detailsArticle XML ↗

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